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Home >> Antibodies >> Cytokeratin 19 (KRT19) (Pancreatic Stem Cell Marker)

Cytokeratin 19 (KRT19) (Pancreatic Stem Cell Marker)

Mouse Monoclonal Antibody [Clone A53-B/A2.26]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded colon carcinoma stained with Cytokeratin 19 Mouse Monoclonal Antibody (A53-B/A2.26).

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Western blot 1) Hep-G2 and 2) MCF-7 cell lysateusing of Cytokeratin 19 Mouse Monoclonal Antibody (A53-B/A2.26).

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Western Blot Analysis of human Prostate Cancer PC-3 cell lysate Cytokeratin 19 Mouse Monoclonal Antibody (A53-B/A2.26).

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SDS-PAGE Analysis Purified Cytokeratin 19 Mouse Monoclonal Antibody (A53-B/A2.26).Confirmation of Integrity and Purity of Antibody.

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Immunofluorescence Analysis of MCF-7 cells labeling Cytokeratin 19 with Cytokeratin 19 Mouse Monoclonal Antibody (A53-B/A2.26) followed by Goat anti-mouse IgG-CF488 (Cyan). The nuclear counterstain is Reddot (Red)

Product Details

Synonyms

k19; k1cs; Keratin 19 Keratin Type i 40kD; krt19

Positive Control

MCF-7, HeLa or Hep-G2 cells, Breast cancer tissue.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.

Immunogen

Human breast cancer MCF-7 cells

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Does not react with Rat or Ferret.

Host / Ig Isotype

Mouse / IgG2a, lambda

Mol. Weight of Antigen

40kDa

Specificity & Comments

This MAb reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40kDa. Its epitope maps between amino acid 312-335. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Karsten et al., Eur.J.Cancer Clin. Oncol. 21(6), 733-740 (1985).

Bioinformatics

  •  Entrez Gene ID: 3880
  •  Gene Symbol: KRT19
  •  SwissProt: P08727
  •  Chromosome Location: 17q21.2
  •  Unigene: 654568
NBT00020