These antibodies perform exceptionally well in the intended applications and have undergone stringent testing and additional validation to ensure monospecificity.
| Catalog Number: | 3880-MSM8 |
|---|---|
| Gene: | |
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Trial Size Available
Carrier-Free Available
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CK19 Mouse Recombinant Monoclonal Antibody (rKRT19/800).
SDS-PAGE Analysis of Purified CK19 Mouse Recombinant Monoclonal Antibody (rKRT19/800). Confirmation of Integrity and Purity of Antibody.
Western Blot Analysis of human lung lysate using CK19 Mouse Recombinant Monoclonal Antibody (rKRT19/800).
Western Blot Analysis of Hep-G2 cell lysate using CK19 Mouse Recombinant Monoclonal Antibody (rKRT19/800).
Western Blot Analysis of Human Skin tissue lysate using Cytokeratin 19 Mouse Recombinant Monoclonal Antibody (rKRT19/800).
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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Recognizes a protein of 40kDa, identified as cytokeratin-19 (CK19), which is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
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