Western Blot Analysis of human Hep-G2 cell lysate using Cytokeratin 19 Recombinant Mouse Monoclonal Antibody (rKRT19/799).
SDS-PAGE Analysis of Purified Cytokeratin 19 Recombinant Mouse Monoclonal Antibody (rKRT19/799). Confirmation of Integrity and Purity of the Antibody.
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 19 Mouse Recombinant Monoclonal Antibody (rKRT19/799).
Flow Cytometric Analysis of PFA-fixed MCF-7 cells using Cytokeratin 19 Mouse Recombinant MAb (rKRT19/799) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 19 Monospecific Recombinant Mouse Monoclonal Antibody (rKRT19/799).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
This MAb reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40kDa. Its epitope maps between amino acid 312-335. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
