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CD81 / TAPA-1

Recombinant Mouse Monoclonal Antibody [Clone rC81/3442]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human tonsil stained with CD81 Recombinant Mouse Monoclonal Antibody (rC81/3442).

Formalin-fixed, paraffin-embedded human tonsil stained with CD81 Recombinant Mouse Monoclonal Antibody (rC81/3442).

Flow cytometry analysis of MCF-7 cells unstained (gray) or stained with CD81 mouse monoclonal antibody (rC81/3442) and goat anti-mouse CF647 (red).

Flow cytometry analysis of bead-bound exosomes derived from MCF-7 cells. Unstained exosomes (gray) or exosomes stained with CF568-labeled CD81 monoclonal antibody (rC81/3442) (orange).

Formalin-fixed, paraffin-embedded human tonsil stained with CD81 Recombinant Mouse Monoclonal Antibody (rC81/3442).

Flow cytometry analysis of MCF-7 cells unstained (gray) or stained with CD81 mouse monoclonal antibody (rC81/3442) and goat anti-mouse CF647 (red).

Flow cytometry analysis of bead-bound exosomes derived from MCF-7 cells. Unstained exosomes (gray) or exosomes stained with CF568-labeled CD81 monoclonal antibody (rC81/3442) (orange).

Product Details

Synonyms

CD81; Target of the antiproliferative antibody 1 (TAPA-1); Tetraspanin-28; Tspan-28

Positive Control

MCF-7, Ramos, Daudi or Jurkat cells. Human lymphocytes. Human tonsil or liver.

Known Applications & Suggested Dilutions

Func., Flow Cyt, IF, Western Blot, IHC-P More Details

Functional Studies (Order Ab without Azide)
Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
Western Blot (1-2ug/ml for 2 hours at RT)
Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human CD81 protein

Cellular Localization

Cell surface

Species Reactivity

Human. Mouse. Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

26kDa

Specificity & Comments

Recognizes a protein of 26kDa, identified as CD81 (Workshop VI; Code CD81.1)). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.