CD81 / TAPA-1 [1.3.3.22]

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Summary

Recognizes a protein of 26kDa, identified as CD81 (Workshop VI; Code CD81.1)). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.

Product Properties & Targets

Host
Species Reactivity
Research Areas
Isotype
Cellular Localization
Gene Name
CD81
Mol. Weight of Antigen
26kDa
Immunogen
Recombinant full-length human CD81 protein

Database Links

Entrez Gene ID
975
Unigene
SwissProt

Additional Information

Clone
1.3.3.22
Human Gene Symbol
CD81
Chromosome Location
11p15.5

Known Applications & Suggested Dilutions

  • Functional Studies (Order Ab without Azide)
  • Flow Cytometry (1-2ug/million cells)
  • Immunofluorescence (1-2ug/ml)
  • Western Blot (1-2ug/ml for 2 hours at RT)
  • Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Key References

  • Kishimoto T et al. eds. Leukocyte Typing VI, p187-189, Garland Publishing, New York, 1997.
  • Takahashi S et al. J Immunol 1990, 145(7):2207-2213.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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