Formalin-fixed, paraffin-embedded human tonsil stained with CD81 Recombinant Mouse Monoclonal Antibody (rC81/3442).

Flow cytometry analysis of MCF-7 cells unstained (gray) or stained with CD81 mouse monoclonal antibody (rC81/3442) and goat anti-mouse CF647 (red).

Flow cytometry analysis of bead-bound exosomes derived from MCF-7 cells. Unstained exosomes (gray) or exosomes stained with CF568-labeled CD81 monoclonal antibody (rC81/3442) (orange).

CD81 / TAPA-1 [rC81/3442]

Name: CD81 / TAPA-1
SKU: 975-MSM3
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies Recombinant Mouse Monoclonals Recombinants

Summary

Recognizes a protein of 26kDa, identified as CD81 (Workshop VI; Code CD81.1)). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.

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Product Properties & Targets

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Isotype

IgG1

Cellular Localization

Basolateral cell membrane, Cell membrane

Gene Name

CD81

Mol. Weight of Antigen

26kDa

Immunogen

Recombinant full-length human CD81 protein

Database Links

Entrez Gene ID

975

Unigene

54457

SwissProt

P60033

Additional Information

Clone

rC81/3442

Human Gene Symbol

CD81

Chromosome Location

11p15.5

Known Applications & Suggested Dilutions

Functional Studies (Order Ab without Azide)
Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
,Western Blot (1-2ug/ml for 2 hours at RT)
,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

Kishimoto T et al. eds. Leukocyte Typing VI, p187-189, Garland Publishing, New York, 1997.
Takahashi S et al. J Immunol 1990, 145(7):2207-2213.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.