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Home >> Antibodies >> CD5 (Mantle Cell Lymphoma Marker)

CD5 (Mantle Cell Lymphoma Marker)

Mouse Monoclonal Antibody [Clone CD5/2418]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Tonsil stained with CD5-Monospecific Mouse Monoclonal Antibody (CD5/2418).

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Purified CD5-Monospecific Mouse Monoclonal Antibody (CD5/2418). Confirmation of Purity and Integrity of Antibody.

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Flow Cytometric Analysis of PFA fixed Ramos Cells. CD5-Monospecific Mouse Monoclonal Antibody (CD5/2418). followed by Goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

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Immunofluorescent staining of PFA fixed Ramos cells using CD5-Monospecific Mouse Monoclonal Antibody (CD5/2418). followed by goat anti-mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using CD5-Monospecific Mouse Monoclonal Antibody (CD5/2418).

Product Details

Synonyms

CD5 antigen (p56 62), LEU1, Ly12, LyA, Lymphocyte antigen T1/Leu-1, Lymphocyte glycoprotein T1/Leu1, T-cell surface glycoprotein CD5

Positive Control

293T, Ramos, or MOLT-4 cells. Tonsil or Lymph Node

Known Applications & Suggested Dilutions

Flow Cyt, Immunohistology         More Details

Flow Cytometry (1-2ug/million cells)
Cells are fixed using 3% PFA.Immunofluorescence (1-2ug/mg)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human CD5 protein (around aa 269-366) (exact sequence is proprietary)

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2c, kappa

Mol. Weight of Antigen

67kDa

Specificity & Comments

Recognizes a 67kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Ferry JA et. al. American Journal of Clinical Pathology, 1996, 105(1):31-7.
  2. Gagneten D et. al. Diagnostic Cytopathology, 1996, 14(1):32-7.

Bioinformatics

  •  Entrez Gene ID: 921
  •  Gene Symbol: CD5
  •  SwissProt: P06127
  •  Chromosome Location: 11q12.2
  •  Unigene: 58685
NBT02418