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Home >> Antibodies >> CD5 (Mantle Cell LymphomaMarker)

CD5 (Mantle Cell LymphomaMarker)

Mouse Monoclonal Antibody [Clone CD5/2418]

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Catalog No Formulation Size Price (USD)  
921-MSM8-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
921-MSM8-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 429.00
921-MSM8-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug
Human Entrez Gene ID 921
Human SwissProt P06127
Human Unigene 58685
Human Gene Symbol CD5
Human Chromosome Location 11q12.2
Synonyms CD5 antigen (p56 62), LEU1, Ly12, LyA, Lymphocyte antigen T1/Leu-1, Lymphocyte glycoprotein T1/Leu1, T-cell surface glycoprotein CD5
Immunogen Recombinant fragment of human CD5 protein (around aa 269-366) (exact sequence is proprietary)
Host / Ig Isotype Mouse / IgG2c, kappa
Mol. Weight of Antigen 67kDa
Cellular Localization Cell surface
Species Reactivity Human. Others not known.

Specificity & Comments

Recognizes a 67kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.

Known Applications & Suggested Dilutions

Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.

Positive Control

293T, Ramos, or MOLT-4 Cells. Tonsil.

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Ferry JA et. al. American Journal of Clinical Pathology, 1996, 105(1):31-7. 2. Gagneten D et. al. Diagnostic Cytopathology, 1996, 14(1):32-7
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