Home >> Antibodies >> CD133 / Prominin (Cancer Stem Cell Marker)

CD133 / Prominin (Cancer Stem Cell Marker)

Mouse Monoclonal Antibody [Clone PROM/6316]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human papillary renal cell carcinoma stained with CD133 / Prominin Mouse Monoclonal Antibody (PROM/6316).

Formalin-fixed, paraffin-embedded human papillary renal cell carcinoma stained with CD133 / Prominin Mouse Monoclonal Antibody (PROM/6316).

SDS-PAGE Analysis Purified CD133 Mouse Monoclonal Antibody (PROM/6316). Confirmation of Integrity and Purity of Antibody.

Formalin-fixed, paraffin-embedded human papillary renal cell carcinoma stained with CD133 / Prominin Mouse Monoclonal Antibody (PROM/6316).

SDS-PAGE Analysis Purified CD133 Mouse Monoclonal Antibody (PROM/6316). Confirmation of Integrity and Purity of Antibody.

Product Details

Synonyms

Antigen AC133; CORD12; Hematopoietic stem cell antigen; hProminin; MCDR2; PROM1; Prominin like protein 1 precursor; PROML1; RP41; STGD4

Positive Control

Raji, HeLa or SW480 cells. Human tonsil.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant chimericPROM1 protein (around aa180-380 and aa612-765). This Mab recognizes both glycosylated and non-glycosylated extracellular domains of CD133.

Cellular Localization

Apical cell membrane. Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1

Mol. Weight of Antigen

97kDa. Depending upon glycosylation, the apparent molecular weight on western blots will increase to ~ 120 kDa.

Specificity & Comments

CD133, also known as PROML1 or Prominin, is a stem cell antigen that may be useful for the selection and expansion of hematopoietic cells and may be used as a positive marker for the characterization of trophoblast cell lines. The human CD133 gene maps to chromosome 4p15.32 and encodes an 865 amino acid protein. The CD133 gene codes for a pentaspantransmembrane glycoprotein that is expressed on primitive hematopoietic stem, progenitor, retinoblastoma, hemangioblasts and neural stem cells and developing epithelium. The 5-TM structure includes an extracellular N-terminus, two short intra- cellular loops, two large extracellular loops and an intracellular C-terminus. CD133 is a candidate gene for retinal proteins that are targeted to plasma membrane protrusions. These retinal proteins, including CD133, may influence the shedding of photoreceptive membranes from the terminal end of the outer segments of vertebrate photoreceptors, where they are phagocytosed by the retinal pigment epithelium, and represent candidates for inherited retinal degenerations.