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CD133 / Prominin (Cancer Stem Cell Marker) Antibody [PROM/6316]

Additional information

Catalog Number: 8842-MSM2
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
8842-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
8842-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
8842-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD133 / Prominin (Cancer Stem Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human papillary renal cell carcinoma stained with CD133 / Prominin Mouse Monoclonal Antibody (PROM/6316).

CD133 / Prominin (Cancer Stem Cell Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human papillary renal cell carcinoma stained with CD133 / Prominin Mouse Monoclonal Antibody (PROM/6316).

CD133 / Prominin (Cancer Stem Cell Marker) Antibody - Image 3

SDS-PAGE Analysis of Purified CD133 Mouse Monoclonal Antibody (PROM/6316). Confirmation of Integrity and Purity of Antibody.

  • Flow
  • IF
  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
PROM/6316
Isotype
IgG1
Cellular Localization
Apical cell membrane, Cell projection, Cilium, Endoplasmic reticulum, Endoplasmic reticulum-Golgi intermediate compartment, Microvillus membrane, Photoreceptor outer segment
Gene Name
PROM1
Positive Control
HeLa or SW480 cells. Human tonsil., Raji
Immunogen
Recombinant chimericPROM1 protein (around aa180-380 and aa612-765). This Mab recognizes both glycosylated and non-glycosylated extracellular domains of CD133.
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).
Key References
  • Ohlfest JR. Journal of immunological methods 361.1-2 (2010 Sep 30): 110-5. (WB, IF, FACS, IHC)
  • Vallera DA. Gynecologic oncology 130.3 (2013 Sep): 579-87.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
8842
SwissProt
O43490
Chromosome Location
4p15.32
Mol. Weight of Antigen
97kDa. Depending upon glycosylation|the apparent molecular weight on western blots will increase to ~ 120 kDa.
Alternate Names
Prominin-1, Antigen AC133, Prominin-like protein 1, Antigen AC133; CORD12; Hematopoietic stem cell antigen; hProminin; MCDR2; PROM1; Prominin like protein 1 precursor; PROML1; RP41; STGD4

Summary

CD133, also known as PROML1 or Prominin, is a stem cell antigen that may be useful for the selection and expansion of hematopoietic cells and may be used as a positive marker for the characterization of trophoblast cell lines. The human CD133 gene maps to chromosome 4p15.32 and encodes an 865 amino acid protein. The CD133 gene codes for a pentaspantransmembrane glycoprotein that is expressed on primitive hematopoietic stem, progenitor, retinoblastoma, hemangioblasts and neural stem cells and developing epithelium. The 5-TM structure includes an extracellular N-terminus, two short intra- cellular loops, two large extracellular loops and an intracellular C-terminus. CD133 is a candidate gene for retinal proteins that are targeted to plasma membrane protrusions. These retinal proteins, including CD133, may influence the shedding of photoreceptive membranes from the terminal end of the outer segments of vertebrate photoreceptors, where they are phagocytosed by the retinal pigment epithelium, and represent candidates for inherited retinal degenerations.

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