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CD133 / Prominin (Cancer Stem Cell Marker) Antibody [PROM/6316]

In Stock
Catalog Number Formulation Size Price
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
Immunohistochemistry (IHC)
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)


CD133, also known as PROML1 or Prominin, is a stem cell antigen that may be useful for the selection and expansion of hematopoietic cells and may be used as a positive marker for the characterization of trophoblast cell lines. The human CD133 gene maps to chromosome 4p15.32 and encodes an 865 amino acid protein. The CD133 gene codes for a pentaspantransmembrane glycoprotein that is expressed on primitive hematopoietic stem, progenitor, retinoblastoma, hemangioblasts and neural stem cells and developing epithelium. The 5-TM structure includes an extracellular N-terminus, two short intra- cellular loops, two large extracellular loops and an intracellular C-terminus. CD133 is a candidate gene for retinal proteins that are targeted to plasma membrane protrusions. These retinal proteins, including CD133, may influence the shedding of photoreceptive membranes from the terminal end of the outer segments of vertebrate photoreceptors, where they are phagocytosed by the retinal pigment epithelium, and represent candidates for inherited retinal degenerations.

Product Properties & Targets

Antibody Type
Species Reactivity
Isotype / Light Chain
IgG1 /
Cellular Localization
Apical cell membrane, Cell projection, Cilium, Endoplasmic reticulum, Endoplasmic reticulum-Golgi intermediate compartment, Microvillus membrane, Photoreceptor outer segment
Gene Name
Positive Control
HeLa or SW480 cells. Human tonsil., Raji
Recombinant chimericPROM1 protein (around aa180-380 and aa612-765). This Mab recognizes both glycosylated and non-glycosylated extracellular domains of CD133.
Alternate Names
Prominin-1, Antigen AC133, Prominin-like protein 1, Antigen AC133; CORD12; Hematopoietic stem cell antigen; hProminin; MCDR2; PROM1; Prominin like protein 1 precursor; PROML1; RP41; STGD4

Database Links

Entrez Gene ID

Additional Information

Chromosome Location
Mol. Weight of Antigen
97kDa. Depending upon glycosylation|the apparent molecular weight on western blots will increase to ~ 120 kDa.


  • May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).

Key References

  • Ohlfest JR. Journal of immunological methods 361.1-2 (2010 Sep 30): 110-5. (WB, IF, FACS, IHC)
  • Vallera DA. Gynecologic oncology 130.3 (2013 Sep): 579-87.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.


This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.


There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.


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