2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> Catenin, gamma (Cardiomyocyte Marker)

Catenin, gamma (Cardiomyocyte Marker)

Mouse Monoclonal Antibody [Clone 15F11]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Western blot analysis: HT20, 293, A431, MCF-7, HepG2 & A549 cell lysates using Catenin, gamma Mouse Monoclonal Antibody (15F11).

img

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using Catenin, gamma Mouse Monoclonal Antibody (15F11) followed by goat anti-mouse IgG-CF488 (green). Nuclei stained with RedDot (red).

img

Western blot analysis of HeLa and SK-BR3 cell lysates using Catenin, gamma Mouse Monoclonal Antibody (15F11).

img

Flow Cytometric Analysis of PFA-fixed MCF-7 cells. Catenin, gamma Mouse Monoclonal Antibody (15F11) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

img

Formalin-fixed, paraffin-embedded human cervix stained with Catenin, gamma Mouse Monoclonal Antibody (15F11). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Product Details

Synonyms

ARVD12; Catenin (cadherin-associated protein), gamma 80kDa; Catenin gamma; CTNNG; Desmoplakin III; Desmoplakin-3; DP3; DPIII; Junction Plakoglobin; PDGB; PKGB

Positive Control

HeLa, SK-BR3 or MCF-7 cells.

Known Applications & Suggested Dilutions

ELISA, Flow Cyt, IF, Western Blot, IHC-P         More Details

ELISA (For coating, order antibody without BSA)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant, full length chicken Catenin gamma protein

Cellular Localization

Cell Surface. Cytoplasm.

Species Reactivity

Human, Mouse, Cat, Rat, Chicken, Cow and Dog.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

80-87kDa

Specificity & Comments

It recognizes a protein of 80-87kDa, identified as gamma-catenin. The catenins (, , and L) are ubiquitously expressed, cytoplasmic proteins that associate with E-cadherin at cellular junctions. Catenin/cadherin complexes play an important role in mediating cellular adhesion. T-catenin, also referred to as VR22, is a 895-amino acid protein that is most abundantly expressed in cardio-myocytes and in the peritubular myoid cells of the testis. T-catenin binds to E-catenin as well as to -catenin, and it functions to inhibit Wnt signaling. CTNNA3, the gene that encodes for -T-catenin, is located on chromosome 10, and mutations in this gene show a strong correlation to late-onset Alzheimer s disease (LOAD) as well as to dilated cardiomyopathy.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Sacco, PA., et al. J Biol Chem 1995, 270:20201-6
  2. Johnson, KR., et al. Exp Cell Res 1993, 207: 252-60

Bioinformatics

  •  Entrez Gene ID: 3728
  •  Gene Symbol: JUP
  •  SwissProt: P14923
  •  Chromosome Location: 17q21
  •  Unigene: 514174