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Catenin, gamma (Cardiomyocyte Marker) Antibody [[11E4]]

In Stock
Catalog Number Formulation Size Price
3728-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3728-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3728-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Western Blot of HT20, A549, 293 and A431 cell lysates using C Catenin, gamma Mouse Monoclonal Antibody (11E4).

Western Blot of HT20, A549, 293 and A431 cell lysates using C Catenin, gamma Mouse Monoclonal Antibody (11E4).

Confocal immunofluorescence image of HeLa cells using Catenin, gamma Mouse Monoclonal Antibody (11E4) Green (CF488) and Reddot is used to label the nuclei Red.

Confocal immunofluorescence image of HeLa cells using Catenin, gamma Mouse Monoclonal Antibody (11E4) Green (CF488) and Reddot is used to label the nuclei Red.

Western Blot Analysis of human HeLa and SKBR3 cell lysate using Catenin, gamma Mouse Monoclonal Antibody (11E4)

Western Blot Analysis of human HeLa and SKBR3 cell lysate using Catenin, gamma Mouse Monoclonal Antibody (11E4)

Flow Cytometric Analysis of PFA-fixed MCF cells. Catenin, gamma Mouse Monoclonal Antibody (11E4) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Flow Cytometric Analysis of PFA-fixed MCF cells. Catenin, gamma Mouse Monoclonal Antibody (11E4) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

It recognizes a protein of 80-87kDa, identified as gamma-catenin. The catenins (, , and ) are ubiquitously expressed, cytoplasmic proteins that associate with E-cadherin at cellular junctions. Catenin/cadherin complexes play an important role in mediating cellular adhesion. T-catenin, also referred to as VR22, is a 895-amino acid protein that is most abundantly expressed in cardio-myocytes and in the peritubular myoid cells of the testis. T-catenin binds to E-catenin as well as to -catenin, and it functions to inhibit Wnt signaling. CTNNA3, the gene that encodes for -T-catenin, is located on chromosome 10, and mutations in this gene show a strong correlation to late-onset Alzheimer s disease (LOAD) as well as to dilated cardiomyopathy.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Western Blot
Species Reactivity
Human, Mouse
Research Areas
Autophagy, Cardiovascular, Developmental Biology, Immunology, Signal Transduction
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Adherens junction, Cell junction, Cytoplasm, Cytoskeleton, Desmosome, Membrane
Gene Name
JUP
Positive Control
293 or A431 cells. Skin., A549, HeLa, HT20, SK-BR3
Immunogen
Recombinant full length human plakoglobin, fused to maltose binding protein
Alternate Names
Junction plakoglobin, Catenin gamma, Desmoplakin III, Desmoplakin-3, ARVD12; Catenin (cadherin-associated protein), gamma 80kDa; Catenin gamma; CTNNG; Desmoplakin III; Desmoplakin-3; DP3; DPIII; Junction Plakoglobin; PDGB; PKGB

Database Links

Entrez Gene ID
3728
SwissProt
P14923

Additional Information

Clone
[11E4]
Chromosome Location
17q21
Mol. Weight of Antigen
80-87kDa

Related Antibodies

View All JUP Antibodies

Functions

  • Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity).

Key References

  • Sacco, PA; McGranahan, TM; Wheelock, MJ; Johnson, KR. Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin. J Biol Chem 1995, 270:20201-6
  • Johnson, KR; Lewis, JE; Li, D; Wahl, J; Soler, AP; Knudsen, KA; Wheelock, MJ. P- and E-cadherin are in separate complexes in cells expressing both cadherins. Exp Cell Res 1993, 207: 252-60

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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