| Catalog Number: | 3728-MSM2 |
|---|---|
| Reactivity: | |
| Gene: | |
| Host: |
Trial Size Available
Carrier-Free Available
Western blot analysis: HT20, 293, A431, MCF-7, HepG2 & A549 cell lysates using Catenin, gamma Mouse Monoclonal Antibody (15F11).
Immunofluorescence Analysis of PFA-fixed HeLa cells stained using Catenin, gamma Mouse Monoclonal Antibody (15F11) followed by goat anti-mouse IgG-CF488 (green). Nuclei stained with RedDot (red).
Western blot analysis of HeLa and SK-BR3 cell lysates using Catenin, gamma Mouse Monoclonal Antibody (15F11).
Flow Cytometric Analysis of PFA-fixed MCF-7 cells. Catenin, gamma Mouse Monoclonal Antibody (15F11) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Formalin-fixed, paraffin-embedded human cervix stained with Catenin, gamma Mouse Monoclonal Antibody (15F11). HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.
SDS-PAGE Analysis of Purified Gamma-Catenin Mouse Monoclonal Antibody (15F11). Confirmation of Purity and Integrity of Antibody.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Flow Cytometry (Flow) | 1-2ug/million cells | Flow Cytometry Protocol | |
| Immunofluorescence (IF) | 1-3ug/ml | ||
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
1
3
3
6
3
6
It recognizes a protein of 80-87kDa, identified as gamma-catenin. The catenins (伪, 尾, 纬 and �L) are ubiquitously expressed, cytoplasmic proteins that associate with E-cadherin at cellular junctions. Catenin/cadherin complexes play an important role in mediating cellular adhesion. 伪 T-catenin, also referred to as VR22, is a 895-amino acid protein that is most abundantly expressed in cardio-myocytes and in the peritubular myoid cells of the testis. 伪 T-catenin binds to 伪 E-catenin as well as to 尾-catenin, and it functions to inhibit Wnt signaling. CTNNA3, the gene that encodes for 伪-T-catenin, is located on chromosome 10, and mutations in this gene show a strong correlation to late-onset Alzheimer s disease (LOAD) as well as to dilated cardiomyopathy.
Should a NeoBiotechnologies antibody not meet the experimental expectations outlined on our website or datasheet, please contact our Technical Support. We unconditionally guarantee our product.
Our team of scientists are ready to help you with expert recommendations or technical assistance related to applications or product use.
Information
© 2026 NeoBiotechnologies.  All Rights Reserved.
Monday-Friday: 8:00am – 8:00pm Pacific Time
