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Home >> Antibodies >> Calnexin (Endoplasmic Reticulum Marker)

Calnexin (Endoplasmic Reticulum Marker)

Mouse Monoclonal Antibody [Clone CANX/1541]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Calnexin Mouse Monoclonal Antibody (CANX/1541).

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Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Calnexin Mouse Monoclonal Antibody (CANX/1541).

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Formalin-fixed, paraffin-embedded human Small Intestinal Carcinoma stained with Calnexin Mouse Monoclonal Antibody (CANX/1541).

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Confocal immunofluorescence image of HeLa cells using Calnexin Mouse Monoclonal Antibody (CANX/1541), labeled in Green. Reddot is used to label the nuclei Red.

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Western Blot Analysis of Human Kidney lysate using Calnexin Mouse Monoclonal Antibody (CANX/1541).

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Western Blot Analysis of HeLa and MCF-7 cell lysate using Calnexin Mouse Monoclonal Antibody (CANX/1541).

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SDS-PAGE Analysis Purified Calnexin Mouse Monoclonal Antibody (CANX/1541). Confirmation of Integrity and Purity of Antibody.

Product Details

Synonyms

Calnexin; CANX; CNX; IP90; Major histocompatibility complex class I antigen-binding protein p88; P90

Positive Control

HeLa, MCF-7 or U2OS cells. Kidney or small intestine.

Known Applications & Suggested Dilutions

ELISA, Western Blot, IHC-P         More Details

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant N-terminal fragment of human Calnexin protein (around aa 1-300) (exact sequence is proprietary)

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Does not react with Rat.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

90kDa

Specificity & Comments

It recognizes a protein of 90kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Williams, D.B. 1995. Calnexin: a molecular chaperone with a taste for carbohydrate. Biochem. Cell Biol. 73: 123-132.

Bioinformatics

  •  Entrez Gene ID: 821
  •  Gene Symbol: CANX
  •  SwissProt: P27824
  •  Chromosome Location: 5q35.3
  •  Unigene: 567968
NBT01541