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Home >> Antibodies >> Bromodeoxyuridine (BrdU) (Proliferation Marker)

Bromodeoxyuridine (BrdU) (Proliferation Marker)

Mouse Monoclonal Antibody [Clone MoBu-1]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analysis Purified BrdU Monoclonal Antibody (MoBu-1). Confirmation of Purity and Integrity of Antibody.

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Formalin-fixed, paraffin-embedded Mouse Small Intestine stained with BrdU Monoclonal Antibody (MoBu-1).

Product Details

Synonyms

Bromodeoxyuridine, BUdr

Positive Control

Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) [For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37oC Optimal dilution for a specific application should be determined.

Immunogen

Bromodeoxyuridine (BrdU) conjugated to hemocyanine (isolated fromHelix pomatia)

Cellular Localization

Nuclear

Species Reactivity

All species

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

Depends on the target

Specificity & Comments

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Harms et al. Acta Histochemica, Suppl. Band 36, 353-359 (1988).
  2. Arras et al. J Clin Invest. 101(1), 40-50 (1998).

Bioinformatics

NBT00884