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T-bet / Tbox-bet / TBX21 Antibody [TBX21/6724]

In Stock
Catalog Number Formulation Size Price
30009-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
30009-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
30009-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
IHC analysis of formalin-fixed, paraffin-embedded human colon carcinoma. T-bet / TBX21 Mouse Monoclonal Antibody (TBX21/6724). Inset: PBS instead of primary antibody; secondary only negative control.

IHC analysis of formalin-fixed, paraffin-embedded human colon carcinoma. T-bet / TBX21 Mouse Monoclonal Antibody (TBX21/6724). Inset: PBS instead of primary antibody; secondary only negative control.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

T helper (Th) lymphocytes differentiate into two unique subsets, Th1 and Th2, which differ both in function and in the cytokines they secrete. Th1 and Th2 cytokines promote the growth and differentiation of their subset, and inhibit the growth and differentiation of the opposing subset. T-bet (T-box expressed in T cells) is a Th1-specific T-box transcription factor that controls the expression of the Th1 cytokine, IFN-g. T-bet also converts effector Th2 cells into the opposing Th1 subset. T-bet is selectively expressed in Th1 cells. The level of T-bet expression is increased by signals mediated by the T cell receptor (TCR). IL-12 also induces an increase in the level of T-bet. T-bet was originally isolated from nuclear extracts of resting and PMA/ionomycin-activated AE7 cells. It is expressed in low levels in AE7 cells, and in increased levels in stimulated AE7.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Nucleus.
Gene Name
TBX21
Positive Control
Human tonsil|non-Hodgkin s lymphoma or spleen. T-cell specific.
Immunogen
Recombinant fragment (around aa 1-200) of human TBX21 protein (exact sequence is proprietary)
Alternate Names
T-box transcription factor TBX21, T-cell-specific T-box transcription factor T-bet, Transcription factor TBLYM, T box expressed in T cells; T box protein 21; T box transcription factor TBX21; T cell specific T box transcription factor T bet; T PET; T-cell-specific T-box transcription factor T-bet; TBET; TBLYM; TPET; Transcription factor TBLYM

Database Links

Entrez Gene ID
30009
SwissProt
Q9UL17

Additional Information

Clone
TBX21/6724
Chromosome Location
17q21.32
Mol. Weight of Antigen
62kDa

Related Antibodies

View All TBX21 Antibodies

Functions

  • Lineage-defining transcription factor which initiates Th1 lineage development from naive Th precursor cells both by activating Th1 genetic programs and by repressing the opposing Th2 and Th17 genetic programs (PubMed:10761931). Activates transcription of a set of genes important for Th1 cell function, including those encoding IFN-gamma and the chemokine receptor CXCR3. Induces permissive chromatin accessibilty and CpG methylation in IFNG (PubMed:33296702). Activates IFNG and CXCR3 genes in part by recruiting chromatin remodeling complexes including KDM6B, a SMARCA4-containing SWI/SNF-complex, and an H3K4me2-methyltransferase complex to their promoters and all of these complexes serve to establish a more permissive chromatin state conducive with transcriptional activation (By similarity). Can activate Th1 genes also via recruitment of Mediator complex and P-TEFb (composed of CDK9 and CCNT1/cyclin-T1) in the form of the super elongation complex (SEC) to super-enhancers and associated genes in activated Th1 cells (PubMed:27292648). Inhibits the Th17 cell lineage commitment by blocking RUNX1-mediated transactivation of Th17 cell-specific transcriptinal regulator RORC. Inhibits the Th2 cell lineage commitment by suppressing the production of Th2 cytokines, such as IL-4, IL-5, and IL- 13, via repression of transcriptional regulators GATA3 and NFATC2. Protects Th1 cells from amplifying aberrant type-I IFN response in an IFN-gamma abundant microenvironment by acting as a repressor of type-I IFN transcription factors and type-I IFN-stimulated genes. Acts as a regulator of antiviral B-cell responses; controls chronic viral infection by promoting the antiviral antibody IgG2a isotype switching and via regulation of a broad antiviral gene expression program (By similarity). Required for the correct development of natural killer (NK) and mucosal-associated invariant T (MAIT) cells (PubMed:33296702).

Key References

  • Paul, W.E. and Seder, R.A. 1994. Lymphocyte responses and cytokines. Cell 76: 241-251.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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