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Recombinant Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Antibody [TOP2A/8103R]

In Stock
Catalog Number Formulation Size Price
7153-RBM10-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
7153-RBM10-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
7153-RBM10-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Antibody

SDS-PAGE Analysis of Purified Topo IIa Recombinant Rabbit Monoclonal Antibody (TOP2A/8103R). Confirmation of Purity and Integrity of Antibody.

Recombinant Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human bladder cancer stained with Topo IIa Recombinant Rabbit Monoclonal Antibody (TOP2A/8103R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Topoisomerase II alpha Antibody in Western Blot (WB)

Topoisomerase II alpha Antibody in Western Blot (WB)

Western Blot Analysis of A549 cell lysate using Topoisomerase II alpha Recombinant Rabbit Monoclonal Antibody (TOP2A/8103R).

Topoisomerase II alpha Antibody Validation in Western Blot on Knockdown(KD) cells.

Topoisomerase II alpha Antibody Validation in Western Blot on Knockdown(KD) cells.

Western Blot validation of anti-Topoisomerase II alpha antibody. Topoisomerase II alpha protein expression was compared in wild-type (WT) and Topoisomerase II alpha shRNA Knockdown (KD) HeLa cells (30ug of total protein). Loading control is shown below.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

The topoisomerase II-α isoform is a 170kDa nuclear protein and plays an important role in DNA synthesis and RNA transcription, as well as chromosomal segregation during mitosis. Topoisomerase II-α is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells, and has been shown to be over-expressed in many human cancers. Decreased expression of Topoisomerase II-α is the predominant mechanism of resistance to several chemotherapeutic agents.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot, Knockdown
Species Reactivity
Human
Research Areas
Nuclear Marker
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Nucleus.
Gene Name
TOP2A
Positive Control
Human tonsil or esophagus. A549
Immunogen
Recombinant fragment (around aa1431-1531) of human Topo II α (TOP2A) protein (exact sequence is proprietary)
Alternate Names
DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme, ATP hydrolyzing DNA topoisomerase II alfa; DNA gyrase; DNA topoisomerase (ATP hydrolyzing); DNA topoisomerase 2 alpha; DNA topoisomerase II 170kD; DNA topoisomerase II alpha; Topoisomerase DNA II alpha 170kDa; TP2A

Database Links

Entrez Gene ID
7153
SwissProt
P11388

Additional Information

Clone
TOP2A/8103R
Chromosome Location
17q21.2
Mol. Weight of Antigen
170kDa

Related Antibodies

View All TOP2A Antibodies

Functions

  • Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612). May play a role in regulating the period length of BMAL1 transcriptional oscillation (By similarity).

Key References

  • Lynch BJ, et al. Hum. Pathol. 1997 Oct;28(10):1180-8
  • Gupta D, et al. Appl. Immunohistochem. Mol. Morphol. 2001 Sep;9(3):215-21.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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