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Recombinant Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Antibody [MSVA-802R]

Additional information

Catalog Number: 7153-RBM12
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
7153-RBM12-P0
Purified Ab with BSA and Azide
20ug
$324.50
7153-RBM12-P1
Purified Ab with BSA and Azide
100ug
$709.50
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
TOP2A Antibody in Numerous Tissues (IHC)

TOP2A Antibody in Numerous Tissues (IHC)

DNA topoisomerase 2-alpha Rabbit Recombinant Monoclonal Antibody (MSVA-802R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1:100-1:200 IHC Protocol Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Product Properties

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
MSVA-802R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cytoplasm, Nucleolus, Nucleoplasm, Nucleus
Gene Name
TOP2A
Positive Control
Colon: A fraction of cells, especially in the crypt basis should show a moderate to strong nuclear TOPO2A immunostaining.
Immunogen
Recombinant human Topoisomerase II alpha fragment (aa1352-1493) (exact sequence is proprietary)
Expression System
HEK293
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612). May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).
Key References
  • Lynch BJ, et al. Hum. Pathol. 1997 Oct;28(10):1180-8
  • Gupta D, et al. Appl. Immunohistochem. Mol. Morphol. 2001 Sep;9(3):215-21.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
7153
SwissProt
P11388
Chromosome Location
17q21.2
Mol. Weight of Antigen
170kDa
Alternate Names
DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme, ATP hydrolyzing DNA topoisomerase II alfa; DNA gyrase; DNA topoisomerase (ATP hydrolyzing); DNA topoisomerase 2 alpha; DNA topoisomerase II 170kD; DNA topoisomerase II alpha; Topoisomerase DNA II alpha 170kDa; TP2A

Summary

The topoisomerase II-伪 isoform is a 170kDa nuclear protein and plays an important role in DNA synthesis and RNA transcription, as well as chromosomal segregation during mitosis. Topoisomerase II-伪 is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells, and has been shown to be over-expressed in many human cancers. Decreased expression of Topoisomerase II-伪 is the predominant mechanism of resistance to several chemotherapeutic agents.

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