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TFAP2A Antibody [PCRP-TFAP2A-2C2]

In Stock
Catalog Number Formulation Size Price
7020-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
7020-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
7020-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

TATA-box binding protein (TBP) interactions with TBP-associated factors (TAFs) are required for the transcription of RNA polymerases. One particular TBP-TAF complex, snRNA-activating protein complex (SNAPC), is unusual in that it regulates basal transcription of both RNA polymerase II and III by binding specifically to a non-TATA-box proximal sequence element (PSE). SNAPC consists of five subunits of varying size. SNAPC binds to Oct-1 and TBP, which are activators of snRNA and RNA polymerases, respectively. The POU domain of Oct-1 binds to SNAPC 190 and effectively recruits SNAPC to the PSE. The cooperative binding of SNAPC and Oct-1 to their respective sequence elements is mediated by a nucleosome positioned between the two sequence elements. SNAPC 19 mediates the assembly of the subunits to form a functional SNAPC transcription regulator. SNAPC 50 (also designated PTFβ) contains two zinc finger motifs and binds to SNAPC 43 (also designated PTFγ) but not SNAPC 45 (PTFδ).

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunohistochemistry
Species Reactivity
Human
Research Areas
Apoptosis, Autophagy, Cardiovascular, Neuroscience, Nuclear Marker, Transcription Factors
Isotype / Light Chain
IgG2a /
Cellular Localization
Nucleus.
Gene Name
TFAP2A
Positive Control
Human skin or placenta. HeLa cells.
Immunogen
Recombinant full-length human TFAP2A protein
Alternate Names
Transcription factor AP-2-alpha, AP-2 transcription factor, Activating enhancer-binding protein 2-alpha, Activator protein 2, AP-2, AP2TF, TFAP2

Database Links

Entrez Gene ID
7020
SwissProt
P05549

Additional Information

Clone
PCRP-TFAP2A-2C2
Chromosome Location
6p24.3
Mol. Weight of Antigen
48kDa

Related Antibodies

View All TFAP2A Antibodies

Functions

  • Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region.

Key References

  • Blackshaw S, et al. Nature methods15.5 (2018 May): 330-338.
  • Statement for PublicationAll investigators should include an acknowledgment in the publications the benefit from the use of this product. Following statement is suggested: PCRP-TFAP2A-2C2 was developed by JHU/CDI under Protein Capture Reagents Program of NICHD of the NIH.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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