Formalin-fixed, paraffin-embedded Rat Pancreas stained with ODC1 Mouse Monoclonal Antibody (ODC1/485).

Formalin-fixed, paraffin-embedded Mouse Pancreas stained with ODC1 Mouse Monoclonal Antibody (ODC1/485).

Western Blot Analysis of human placental lysate using ODC-1 Mouse Monoclonal Antibody (ODC1/485).

SDS-PAGE Analysis Purified ODC-1 Mouse Monoclonal Antibody (ODC1/485). Confirmation of Purity and Integrity of Antibody.

Flow Cytometric Analysis of human ODC-1 in PC3 cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled ODC-1 Mouse Monoclonal Antibody (ODC1/485).

Confocal immunofluorescence image of LNCaP cells using AF488 labeled ODC-1 Mouse Monoclonal Antibody (ODC1/485) (Green). Nuclei are counter stained with DAPI (Blue).

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with ODC-1 Mouse Monoclonal Antibody (ODC1/485).

Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with ODC-1 Mouse Monoclonal Antibody (ODC1/485).

Western Blot Analysis of PC3 cell lysate using ODC-1 Mouse Monoclonal Antibody (ODC1/485).

Ornithine Decarboxylase-1 (ODC-1) [ODC1/485]

Name: Ornithine Decarboxylase-1 (ODC-1)
SKU: 4953-MSM1
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies

Summary

Recognizes a 53kDa protein, identified as the Ornithine Decarboxylase (ODC-1). ODC is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. ODC activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.

Printable

Product Properties & Targets

Host

Mouse

Species Reactivity

Human

Isotype

IgG1

Gene Name

ODC1

Mol. Weight of Antigen

53kDa.

Immunogen

Recombinant full-length human ODC-1 protein

Database Links

Entrez Gene ID

4953

Unigene

467701

SwissProt

P11926

Additional Information

Clone

ODC1/485

Human Gene Symbol

ODC1

Chromosome Location

2p25.1

Known Applications & Suggested Dilutions

Western Blot (0.5-1ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Key References

Schipper RG; et al. Journal of Immunological Methods, 1993, 161(2):205-15.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.