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Recombinant MGMT (Marker of Suppressor of Mutation and Carcinogenesis) Antibody [MGMT/8364R]

In Stock
Catalog Number Formulation Size Price
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)


Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal crossâ � �links at the O6â � � alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6â � �position of guanine in DNA (prior to crossâ � �link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible; hence the level of MGMT in a cell is directly proportional to the level of DNAâ � �damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant

Product Properties & Targets

Species Reactivity
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Gene Name
Positive Control
Jurkat or MCF-7 cells. Human tonsil or stomach (IHC).
Recombinant fragment (around aa1-100) of human MGMT (exact sequence is proprietary)
Alternate Names
Methylated-DNA | protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase, 6 O methylguanine DNA methyltransferase; Methylated DNA protein cysteine methyltransferase; O 6 methylguanine DNA alkyltransferase; O 6 methylguanine DNA methyltransferase

Database Links

Entrez Gene ID

Additional Information

Chromosome Location
Mol. Weight of Antigen
21.646 kDa


  • Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Key References

  • D Incalci, M., et al. 1988. Importance of the DNA repair enzyme O6-alkylguanine alkyltransferase (AT) in cancer chemotherapy. Cancer Treat. Rev.15: 279-292.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.


This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.


There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.


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