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Formalin-fixed, paraffin-embedded human testis stained with MGMT Mouse Monoclonal Antibody (MGMT/7454). Inset: PBS instead of primary antibody; secondary only negative control.
Formalin-fixed, paraffin-embedded human salivary gland stained with MGMT Mouse Monoclonal Antibody (MGMT/7454). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
SDS-PAGE Analysis of Purified MGMT Mouse Monoclonal Antibody (MGMT/7454). Confirmation of Purity and Integrity of Antibody.
Western blot analysis of MCF-7 cell lysate using MGMT Mouse Monoclonal Antibody (MGMT/7454).
Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal cross �links at the O6 � alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6 �position of guanine in DNA (prior to cross �link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible; hence the level of MGMT in a cell is directly proportional to the level of DNA �damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant
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