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MBD1 (Methyl-CpG Binding Domain Protein 1) Antibody [CPTC-MBD1-1]

In Stock
Catalog Number Formulation Size Price
4152-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4152-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4152-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human colon stained with Methyl-CpG Binding Domain Protein 1 Mouse Monoclonal Antibody (CPTC-MBD1-1)

Formalin-fixed, paraffin-embedded human colon stained with Methyl-CpG Binding Domain Protein 1 Mouse Monoclonal Antibody (CPTC-MBD1-1)

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. MBD1 has also been predicted to be involved in the development and progression of tumors, and it has been shown that MBD1 is significantly upregulated in pancreatic cancer tissues when compared with surrounding normal tissues. Furthermore, MBD1 may serve as a potential therapeutic target for pancreatic cancer.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Nuclear Marker
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Chromosome, Nucleus, Nucleus matrix, Nucleus speckle
Gene Name
MBD1
Positive Control
Human tonsil or colon (IHC).
Immunogen
Recombinant human MBD1 protein
Alternate Names
Methyl-CpG-binding domain protein 1, CXXC-type zinc finger protein 3, Methyl-CpG-binding protein MBD1, Protein containing methyl-CpG-binding domain 1, CXXC-type zinc finger protein 3; CXXC3; MBD1; MECP1 COMPLEX; Methyl-CpG-binding domain protein 1 (MBD1); Protein containing methyl-CpG-binding domain 1 (PCM1); Regulator of fibroblast growth factor 2 (FGF 2) transcription (RFT)

Database Links

Entrez Gene ID
4152
SwissProt
Q9UIS9

Additional Information

Clone
CPTC-MBD1-1
Chromosome Location
18q21
Mol. Weight of Antigen
~80kDa

Related Antibodies

View All MBD1 Antibodies

Functions

  • Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting ATF7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.

Key References

  • Hendrich, B. and Bird, A. 1998. Identification and characterization of a family of mammalian methyl-CpG binding proteins. Mol. Cell. Biol. 18: 6538-6547.
  • Ng, H.H., et al. 1999. MBD2 is a transcriptional repressor belonging to the MeCP1 histone deacetylase complex. Nat. Genet. 23: 58-61.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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