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HPV16 E1^E4 (Human Papilloma Virus 16) Antibody [HPV16 E1/E4]

In Stock
Catalog Number Formulation Size Price
MSM9-3066-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
MSM9-3066-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
MSM9-3066-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
HPV16 Antibody in Immunohistochemistry (IHC (P))

HPV16 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human Cervix stained with HPV-16 Mouse Monoclonal Antibody (HPV16 E1/E4).

HPV16 Antibody in Immunohistochemistry (IHC (P))

HPV16 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human Cervix stained with HPV-16 Mouse Monoclonal Antibody (HPV16 E1/E4).

HPV16 Antibody in SDS-PAGE

HPV16 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified HPV-16 Mouse Monoclonal Antibody (HPV16 E1/E4). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

The human papilloma virus (HPV) family of DNA tumor viruses includes HPV16, a “high-risk” sexually-transmitted HPV that can lead to cervical, anal, vulvar, head, neck, and penile cancer. The HPV16E1/E4 protein is expressed abundantly in cells supporting viral DNA amplification but is lost during malignant progression. HPV16E1/E4 causes G2 cell cycle arrest by associating with and preventing the nuclear entry of Cdk1/cyclin B1 complexes. HPV16E1/E4 also interacts with cyclin A and Cdk2 during the G2 phase of the cell cycle, and this association may increase the efficiency with which HPV16E1/E4 is able to prevent mitotic entry. HPV16E1/E4 also associates with keratin intermediate filaments and causes the network to collapse.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
HPV-16
Research Areas
Cancer, Infectious Disease, Ovarian Cancer
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Host cytoplasm, Host nucleus
Gene Name
E1^E4
Positive Control
HPV-16 E1^E4 infected cells. Cervical tissue.
Immunogen
Recombinant human HPV16 E1/E4 fragment (aa 36-41).
Alternate Names
Protein E4, E1^E4, cell cycle modulating protein E1^E4; HPV-16 E1-E4; Replication protein E1; Replication protein E4; Human papillomavirus type 16 E1^E4

Database Links

Entrez Gene ID
1489076
SwissProt
P06922

Additional Information

Clone
HPV16 E1/E4
Chromosome Location
Not Applicable
Mol. Weight of Antigen
16kDa

Related Antibodies

View All E1^E4 Antibodies

Functions

  • N/A

Key References

  • Reich, N.C., et al. 1983. Mol. Cell. Biol. 3: 2143-2150.
  • Doorbar J. (2013). The E4 protein; structure, function and patterns of expression. Virology, 445(1-2), 80–98. https://doi.org/10.1016/j.virol.2013.07.008
  • Davy, C. E., et al. (2006). HPV16 E1--E4 protein is phosphorylated by Cdk2/cyclin A and relocalizes this complex to the cytoplasm. Virology, 349(1), 230–244. https://doi.org/10.1016/j.virol.2006.02.024
  • Doorbar, J., et al. (1991). Specific interaction between HPV-16 E1-E4 and cytokeratins results in collapse of the epithelial cell intermediate filament network. Nature, 352(6338), 824–827. https://doi.org/10.1038/352824a0
  • Doorbar, J., et al. (1992). Epitope-mapped monoclonal antibodies against the HPV16E1--E4 protein. Virology, 187(1), 353–359. https://doi.org/10.1016/0042-6822(92)90327-l

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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