DOG-1 / TMEM16A (Gastrointestinal Stromal Tumor Marker) [DG1/447]

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Summary

Expression of DOG-1 protein is elevated in the gastrointestinal stromal tumors (GISTs is nearly identical: 94.4% vs. 94.7%.

Product Properties & Targets

Host
Species Reactivity
Isotype
Cellular Localization
Cell Surface and Cytoplasm
Gene Name
Positive Control
Gastrointestinal Stromal Tumor (GIST) or testicular germ cell tumor. Melanocytes in the basal layer of the epidermis and mast cells in the dermis of normal skin.
Immunogen
Recombinant human DOG-1 protein
Alternate Names
V-set domain-containing T-cell activation inhibitor 1, B7 homolog 4, B7h.5, Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, Anoctamin 1, Calcium Activated Chloride Channel, Discovered On Gastrointestinal Stromal Tumors Protein 1,TAOS2, ORAOV2, TMEM16A

Database Links

Entrez Gene ID
Unigene
SwissProt

Additional Information

Clone
DG1/447
Human Gene Symbol
TMEM16A
Chromosome Location
11q13.3
Mol. Weight of Antigen
~114kDa

Functions

  • Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.

Known Applications & Suggested Dilutions

  • Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood);Immunofluorescence (1:50-1:100);Optimal dilution for a specific application should be determined.

Key References

  • Espinosa I, et. al. Am J Surg Pathol 2008;32:210-218.

Storage & Stability

Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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