Formalin-fixed, paraffin-embedded human Skin stained with Multi-Cytokeratin Recombinant Rabbit Monoclonal Antibody (KRT/1877R).

SDS-PAGE Analysis Purified Multi-Cytokeratin Rabbit Monoclonal Antibody (KRT/1877R). Confirmation of Integrity and Purity of Antibody.

Immunofluorescence Analysis of MeOH-fixed Human MCF-7 cells labeling CK with Multi-Cytokeratin Recombinant Rabbit Monoclonal Antibody (KRT/1877R)followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

Cytokeratin, Multi (Epithelial Marker) [KRT/1877R]

Name: Cytokeratin, Multi (Epithelial Marker)
SKU: RBM37-1877
Availability: InStock

Monoclonals Primary Antibodies Rabbit Monoclonals Recombinant Rabbit Monoclonals Recombinants

Summary

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 53kDa (CK13) and 45kDa (CK18). This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Printable

Product Properties & Targets

Host

Rabbit

Species Reactivity

Cow, Frog, Goat, Guinea Pig, Human, Monkey, Mouse, Pig, Rat

Isotype

IgG

Gene Name

KRT6A

Mol. Weight of Antigen

Multiple

Immunogen

Keratin-enriched preparation from cultured human epithelial cells

Database Links

Entrez Gene ID

3851; 3852; 3853; 3854; 286887; 3856; 3858; 3860; 3875

Unigene

SwissProt

P02538 ; P04259 ; P13647 ; P19013 ; P48668

Additional Information

Clone

KRT/1877R

Known Applications & Suggested Dilutions

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Key References

Bartek J et. al. J Pathol, 1991, 164(3):215-24.
Kasper M. Histochemistry, 1991, 95(6):613-20.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.