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Recombinant Cytokeratin 6A (KRT6A) (Basal Cell Marker) Antibody [rKRT6A/2100]

In Stock
Catalog Number Formulation Size Price
3853-MSM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3853-MSM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3853-MSM6-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

This MAb recognizes a protein of 56kDa, identified as cytokeratin 6A (KRT6A). In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns. Cytokeratin 6A is the dominant form in epithelial tissue. Cytokeratin 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Cytokeratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express KRT6 and KRT16. KRT6 is strongly expressed in about 75% of head and neck squamous cell carcinomas.

Product Properties & Targets

Host
Mouse
Applications
Species Reactivity
Research Areas
Isotype / Light Chain
IgG1 / Kappa
Gene Name
Positive Control
Tonsil or basal cell carcinoma.
Immunogen
Recombinant full-length human Cytokeratin 6A (KRT6A) protein
Alternate Names
Keratin, type II cytoskeletal 6A, Cytokeratin-6A, Cytokeratin-6D, Keratin-6A, Type-II keratin Kb6, 58kDa Cytokeratin; CK5; Cytokeratin-5; DDD1; Epidermolysis Bullosa Simplex 2 (EBS2); Keratin 5; Keratin, Type II Cytoskeletal 5; Keratin-5; KRT5; Type-II Cytoskeletal 5; Type-II keratin Kb5

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
rKRT6A/2100
Chromosome Location
12q13.13
Mol. Weight of Antigen
58kDa

Functions

  • Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair.

Key References

  • Stephens, K., et al. 1995. Am. J. Hum. Genet. 56: 577-5

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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