Learn about our comprehensive antibody validation methods to ensure monospecificity.  Antibody Validation>>

Recombinant Cytokeratin 6A (KRT6A) (Basal Cell Marker) Antibody [KRT6/3997R]

In Stock
Catalog Number Formulation Size Price
3853-RBM7-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3853-RBM7-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3853-RBM7-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Cytokeratin 6 is an epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. This protein always paired with the type-I keratin K16.Cytokeratin 6 have been identified in epidermis only in samples from plantar glabrous skin while in hairy skin this keratin appeared to be absent in interfollicular epidermis but were clearly present in hair follicle outer root sheath, and this protein also consistently expressed in non-keratinizing stratified squamous epithelia. Among tumors, cytokeratin 6 is typically and strongly expressed in squamous cell carcinomas of different sites, preferentially in inner, maturing layers of the tumor cell nests.Cytokeratin 6 is always used with cytokeratin 5 and is a useful aid for classification of epithelioid mesotheliomas.

Product Properties & Targets

Host
Rabbit
Applications
Species Reactivity
Research Areas
Isotype / Light Chain
IgG / Kappa
Gene Name
Positive Control
HeLa cells. Human tonsil or basal cell carcinoma (BCC).
Immunogen
Recombinant fragment of human KRT6 (exact sequence is proprietary)
Alternate Names
Keratin, type II cytoskeletal 6A, Cytokeratin-6A, Cytokeratin-6D, Keratin-6A, Type-II keratin Kb6, CK6A, CK6B, CK6C, CK6D, CK6E, Keratin Type II Cytoskeletal 6A, Keratin Type II Cytoskeletal 6B, Keratin Type II

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
KRT6/3997R
Chromosome Location
12q13.13
Mol. Weight of Antigen
56kDa

Functions

  • Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair.

Key References

  • Stephens, K., et al. 1995. Epidermolysis bullosa simplex: a keratin 5 mutation is a fully dominant allele in epidermal cytoskeleton function. Am. J. Hum. Genet. 56: 577-585.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Recombinant Cytokeratin 6A (KRT6A) (Basal Cell Marker) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

We hold Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK