Learn about our comprehensive antibody validation methods to ensure monospecificity.  Antibody Validation>>

Printable Datasheet

Cytokeratin, Multi (Epithelial Marker) Antibody [KRT/457]

In Stock
Catalog Number Formulation Size Price
MSM15-457-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
MSM15-457-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
MSM15-457-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Multi Cytokeratin Monoclonal Antibody (KRT/457).

Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Multi Cytokeratin Monoclonal Antibody (KRT/457).

Formalin-fixed, paraffin-embedded Rat Oviduct stained with Multi Cytokeratin Monoclonal Antibody (KRT/457).

Formalin-fixed, paraffin-embedded Rat Oviduct stained with Multi Cytokeratin Monoclonal Antibody (KRT/457).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 53kDa (CK13) and 45kDa (CK18). This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Cow, Frog, Goat, Guinea Pig, Human, Monkey, Mouse, Pig, Rat
Isotype / Light Chain
IgG1 / Kappa
Gene Name
KRT6A
Positive Control
A431 cells, Colon carcinoma, Skin
Immunogen
Keratin-enriched preparation from cultured human epithelial cells
Alternate Names
Keratin, type II cytoskeletal 6A, Cytokeratin-6A, Cytokeratin-6D, Keratin-6A, Type-II keratin Kb6

Database Links

Entrez Gene ID
3851; 3852; 3853; 3854; 286887; 3856; 3858; 3860; 3875
SwissProt
P02538 ; P04259 ; P13647 ; P19013 ; P48668

Additional Information

Clone
KRT/457
Mol. Weight of Antigen
Multiple

Related Antibodies

View All KRT6A Antibodies

Functions

  • Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair.

Key References

  • Bartek J et. al. J Pathol, 1991, 164(3):215-24.
  • Kasper M. Histochemistry, 1991, 95(6):613-20.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Cytokeratin, Multi (Epithelial Marker) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

We hold Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales