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Recombinant Cyclin B1 (G2- & M-phase Cyclin) Antibody [CCNB1/7030R]

In Stock
Catalog Number Formulation Size Price
891-RBM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
891-RBM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
891-RBM46-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Summary

It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

Product Properties & Targets

Host
Rabbit
Applications
Species Reactivity
Research Areas
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Centrosome, Cytoplasm, Cytoskeleton, Microtubule organizing center, Nucleus
Gene Name
Positive Control
Cell line in logarithmic growth phase. Human tonsil, Prostate or Ovarian Carcinoma.
Immunogen
Recombinant full-length human CCNB1 protein
Alternate Names
G2/mitotic-specific cyclin-B1, CCNB, CCNB1, CCNB1_HUMAN, G2 Mitotic Specific Cyclin B1

Database Links

Entrez Gene ID
891
SwissProt

Additional Information

Clone
CCNB1/7030R
Chromosome Location
5q13.2
Mol. Weight of Antigen
55-62kDa

Functions

  • Essential for the control of the cell cycle at the G2/M (mitosis) transition.

Key References

  • Garner AP et al. Oncogene21:8089-104 (2002).
  • Minemoto Y et al. Exp Cell Res262:37-48 (2001).

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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