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Formalin-fixed, paraffin-embedded human Tonsil stained with Cyclin B1 Mouse Monoclonal Antibody (CCNB1/1098).
Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Cyclin B1 Mouse Monoclonal Antibody (CCNB1/1098).
Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with Cyclin B1 Mouse Monoclonal Antibody (CCNB1/1098).
Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cyclin B1 Mouse Monoclonal Antibody (CCNB1/1098).
Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Cyclin B1 Mouse Monoclonal Antibody (CCNB1/1098).
SDS-PAGE Analysis Purified Cyclin B1 Mouse Monoclonal Antibody (CCNB1/1098). Confirmation of Integrity and Purity of Antibody.
MCF-7 cells stained with CF555 Cyclin B1 Mouse Monoclonal Antibody (CCNB1/1098) (nuclei/nucleoli, red) and CF488A phalloidin (actin filaments, green). DNA is stained with Hoechst (blue).
Immunofluorescence Analysis of formaldehyde-fixed MCF-7 cells stained with 5 ug/mL CF568 Mouse anti-Cyclin B1 (CCNB1/1098) (red) and Hoechst (blue).
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
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It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
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