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Cyclin B1 (G2- & M-phase Cyclin) Antibody [V92.1]

Additional information

Catalog Number: 891-MSM1
Reactivity:

, ,

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
891-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
891-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
891-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Cyclin B1 (G2- & M-phase Cyclin) Antibody

SDS-PAGE Analysis of Purified Cyclin B1 Mouse Monoclonal Antibody (V92.1). Confirmation of Integrity and Purity of Antibody.

Cyclin B1 (G2- & M-phase Cyclin) Antibody - Image 2

Immunofluorescence Analysis of PFA fixed HeLa cells labeling Cyclin B1 Mouse Monoclonal Antibody (V92.1) followed by Goat anti-mouse IgG-CF488 (Green).

Cyclin B1 (G2- & M-phase Cyclin) Antibody - Image 3

Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells using Cyclin B1 Mouse Monoclonal Antibody (V92.1) followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

  • Flow
  • IF
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence
Species Reactivity
Hamster, Human, Mouse
Clone
V92.1
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Centrosome, Cytoplasm, Cytoskeleton, Microtubule organizing center, Nucleus
Gene Name
CCNB1
Positive Control
Cell line in logarithmic growth phase. Tonsil or testicular, Endometrial, Prostate or Ovarian Carcinoma.
Immunogen
Hamster Cyclin B1 protein
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Key References
  • Garner AP et al. Oncogene 21:8089-104 (2002)
  • Minemoto Y et al. Exp Cell Res 262:37-48 (2001)
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
891
SwissProt
P14635
Chromosome Location
5q13.2
Mol. Weight of Antigen
55-62kDa
Alternate Names
G2/mitotic-specific cyclin-B1, CCNB, CCNB1, CCNB1_HUMAN, G2 Mitotic Specific Cyclin B1

Summary

It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

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