| Catalog Number: | 1294-MSM1 |
|---|---|
| Reactivity: | |
| Gene: | |
| Host: |
Trial Size Available
Carrier-Free Available
Formalin-fixed, paraffin-embedded human Skin stained with Collagen VII Mouse Monoclonal Antibody (LH7.2).
Formalin-fixed, paraffin-embedded human Skin stained with Collagen VII Mouse Monoclonal Antibody (LH7.2).
SDS-PAGE Analysis of Purified Collagen VII Mouse Monoclonal Antibody (LH7.2). Confirmation of Purity and Integrity of Antibody.
Immunofluorescence Analysis of PFA-fixed HeLa cells labeling Collagen VII using Collagen VII Mouse Monoclonal Antibody (LH7.2) followed by goat anti-mouse IgG-CF488 (Green). The nuclear counterstain is RedDot.
Flow Cytometric Analysis of PFA-fixed HeLa cells using Collagen VII Mouse Monoclonal Antibody (LH7.2) followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Flow Cytometry (Flow) | 1-2ug/million cells | Flow Cytometry Protocol | |
| Immunofluorescence (IF) | 1-3ug/ml | ||
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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Recognizes an epitope located on collagenase digested Type VII collagen (150kDa), i.e. the non-helical carboxyl terminal region of the dimer. Binding is localized at the inferior border of the lamina densa as demonstrated by immunoelectron microscopy. Type VII is also found in the retina, where its function is unknown. It interacts with Laminin 5 and Fibronectin. Collagen VII is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
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