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Collagen VII Antibody [LH7.2]

In Stock
Catalog Number Formulation Size Price
1294-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1294-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1294-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

Recognizes an epitope located on collagenase digested Type VII collagen (150kDa), i.e. the non-helical carboxyl terminal region of the dimer. Binding is localized at the inferior border of the lamina densa as demonstrated by immunoelectron microscopy. Type VII is also found in the retina, where its function is unknown. It interacts with Laminin 5 and Fibronectin. Collagen VII is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Research Areas
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Basement membrane, Extracellular matrix, Extracellular space, Secreted
Gene Name
Positive Control
cells. Human lymphocytes. Human lymph node or tonsil., HeLa
Immunogen
Recombinant full-length human Collagen VII protein
Alternate Names
Collagen alpha-1(VII) chain, Long-chain collagen, Alpha 1 type VII collagen; Collagen 7; Collagen alpha 1(VII) chain; Collagen type VII alpha 1; Collagen VII alpha 1 polypeptide Col7a1; EBD1; EBDCT; EBR1; Long-chain (LC) collagen

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
LH7.2
Chromosome Location
3p21.31
Mol. Weight of Antigen
290kDa

Functions

  • Stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.

Key References

  • Ponsioen TL et al. Mol Vis 14:652-60 (2008).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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