Immunofluorescentstaining of PFA-fixed Jurkatcells using CD28Mouse Monoclonal Antibody (CB28) followed by goat anti-mouse IgG-CF488 (green). Nuclei stained by RedDot (red).

Flow Cytometric Analysis of PFA-fixedJurkatcellsusing CD28Mouse Monoclonal Antibody (CB28)followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

SDS-PAGE Analysis Purified CD28Mouse Monoclonal Antibody (CB28) Confirmation of Integrity and Purity of Antibody.

Printable Datasheet

CD28 [CB28]

Name: CD28
SKU: 940-MSM1
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies

Summary

Recognizes a glycoprotein of 44-88kDa, which is identified as CD28. Itis the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

Product Properties & Targets

Host

Mouse

Applications

Flow Cytometry, Functional Studies, Immunofluorescence

Species Reactivity

Human

Isotype

IgG1

Cellular Localization

Cell surface, Membrane

Gene Name

CD28

Positive Control

Jurkat cells. Human lymph nodes and tonsils.

Immunogen

Recombinant human CD28 protein

Alternate Names

T-cell-specific surface glycoprotein CD28, TP44, T-cell-specific surface glycoprotein CD28; Tp44

Database Links

Entrez Gene ID

940

Unigene

591629

SwissProt

P10747

Additional Information

Clone

CB28

Human Gene Symbol

CD28

Chromosome Location

2q33.2

Mol. Weight of Antigen

44kDa (monomer); 88kDa (dimer)

Functions

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933). Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037).

Known Applications & Suggested Dilutions

Flow Cytometry (1-2ug/million cells)
,Immunofluorescence (1-2ug/ml)
,Functional Studies (Order Ab without BSA & Azide)
,Optimal dilution for a specific application should be determined.

Key References

Scharschmidt E, et al. Mol Cell Biol. 2004;24(9):3860-73.
Jeong SH, et al. J Virol. 2004;78:6995-7003.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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