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CD28 Antibody [CB28]

Name: CD28 Antibody
SKU: 940-MSM1-PE
Price: 519.00 USD
Availability: InStock

In Stock

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SDS-PAGE Analysis of Purified CD28 Mouse Monoclonal Antibody (CB28). Confirmation of Integrity and Purity of Antibody.

Applications & Dilutions

Flow Cytometry (Flow)

1-2ug/million cells

Flow Cytometry Protocol

Immunofluorescence (IF)

1-3ug/ml

Summary

Recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

Product Properties & Targets

Antibody Type

Mouse Monoclonals

Host

Mouse

Applications

Flow Cytometry, Immunofluorescence

Species Reactivity

Human

Research Areas

Cancer, Cardiovascular, Immuno Oncology, Immunology, AKT Signaling, Dendritic Cell Marker, Hematopoietic Stem Cells, Immune checkpoint, Infectious Disease, Signal Transduction

Isotype / Light Chain

IgG1 / Kappa

Cellular Localization

Cell surface

Gene Name

CD28

Positive Control

Jurkat cells. Human lymph nodes and tonsils.

Immunogen

Recombinant human CD28 protein

Alternate Names

T-cell-specific surface glycoprotein CD28, TP44, T-cell-specific surface glycoprotein CD28; Tp44

Database Links

Entrez Gene ID

940

SwissProt

P10747

Additional Information

Clone

CB28

Chromosome Location

2q33.2

Mol. Weight of Antigen

44kDa (monomer); 88kDa (dimer)

Functions

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933). Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037).

Key References

Scharschmidt E, Wegener E, Heissmeyer V, Rao A, Krappmann D: Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling. Mol Cell Biol. 2004;24(9):3860-73.
Jeong SH, Qiao M, Nascimbeni M, Hu Z, Rehermann B, Murthy K, Liang TJ. Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates. J Virol. 2004;78:6995-7003.

PubMed Links

Storage & Stability

Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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