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Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD28 Mouse Monoclonal Antibody (CB28) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
SDS-PAGE Analysis of Purified CD28 Mouse Monoclonal Antibody (CB28) (unconjugated). Confirmation of Integrity and Purity of Antibody.
Immunofluorescent staining of PFA-fixed Jurkat cells. CD28 Mouse Monoclonal Antibody (CB28) followed by goat anti-mouse IgG-CF488 (green). Nuclei stained by RedDot (red).
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol |
|---|---|---|
| Flow Cytometry (Flow) | 1-2ug/million cells | Flow Cytometry Protocol |
| Immunofluorescence (IF) | 1-3ug/ml |
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Recognizes a glycoprotein of 44-88kDa, which is identified as CD28. Itis the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
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