Learn about our comprehensive antibody validation methods to ensure monospecificity.  Antibody Validation>>

CD28 Antibody [CB28]

In Stock
Catalog Number Formulation Size Price
940-MSM1-PE-100T
Purified Ab Conjugated to PE
0.5ml at 100ug/ml
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml

Summary

Recognizes a glycoprotein of 44-88kDa, which is identified as CD28. Itis the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG1 /
Cellular Localization
Cell surface, Membrane
Gene Name
Positive Control
Jurkat cells. Human lymph nodes and tonsils.
Immunogen
Recombinant human CD28 protein
Alternate Names
T-cell-specific surface glycoprotein CD28, TP44, T-cell-specific surface glycoprotein CD28; Tp44

Database Links

Entrez Gene ID
940
SwissProt

Additional Information

Clone
CB28
Chromosome Location
2q33.2
Mol. Weight of Antigen
44kDa (monomer); 88kDa (dimer)

Functions

  • Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933). Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037).

Key References

  • Scharschmidt E, et al. Mol Cell Biol. 2004;24(9):3860-73.
  • Jeong SH, et al. J Virol. 2004;78:6995-7003.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “CD28 Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

We hold Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK