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CD203c / ENPP3 (Basophil Marker) Antibody [ENPP3/9892]

Additional information

Catalog Number: 5169-MSM2
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
5169-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
5169-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
5169-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD203c Antibody in Immunohistochemistry (IHC (P))

CD203c Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human kidney stained with CD203c / ENPP3 Mouse Monoclonal Antibody (ENPP3/9892). Inset: PBS instead of primary antibody; secondary only negative control.

CD203c Antibody in SDS-PAGE

CD203c Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CD203c Mouse Monoclonal Antibody (ENPP3/9892). Confirmation of Purity and Integrity of Antibody.

ENPP3 Antibody in Western Blot (WB)

ENPP3 Antibody in Western Blot (WB)

Western blot analysis of kidney tissue lysates from human, mouse, hamster and rabbit using ENPP3 Mouse Monoclonal Antibody (ENPP3/9892).

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
ENPP3/9892
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Apical cell membrane, Cell membrane, Secreted
Gene Name
ENPP3
Positive Control
Kidney.
Immunogen
Recombinant fragment (around aa300-600) of human ENPP3 protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Host:

Formalin-fixed, paraffin-embedded human Colon stained with CDH17-Monospecific Mouse Monoclonal Antibody (CDH17/2615).
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Host:

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Host:

Reactivity:

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Cleaves a variety of phosphodiester and phosphosulfate bonds including deoxynucleotides, nucleotide sugars, and NAD.
Key References
  • Binder, M., et al. 2002. Individual hymenoptera venom compounds induce upregulation of the basophil activation marker ectonucleotide pyrophosphatase/phosphodiesterase 3 (CD203c) insensitized patients. Int. Arch. Allergy Immunol. 129: 160-168.
  • Yano, Y., et al. 2003. Expression and localization of ecto-nucleotide pyrophosphatase/phosphodiesterase I-3 (E-NPP3/CD203c/PD-Ib/B10/gp130RB13-6) in human colon carcinoma. Int. J. Mol. Med. 12: 763-766.
  • Majlesi, Y., et al. 2003. Cerivastatin and atorvastatin inhibit IL-3-dependent differentiation and IgE-mediated histamine release in human basophils and downmodulate expression of the basophil-activation antigen CD203c/E-NPP3. J. Leukoc. Biol. 73: 107-117.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
5169
SwissProt
O14638
Chromosome Location
6q23.2
Mol. Weight of Antigen
100kDa
Alternate Names
ectonucleotide pyrophosphatase/phosphodiesterase family member 3, Alkaline phosphodiesterase I antibody; ARHR2 antibody; B10 antibody; CD203c antibody; CD203c antigen antibody; dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) antibody dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) antibody; E NPP 3 antibody; E-NPP 3 antibody; Ectonucleotide pyrophosphatase/phosphodiesterase 3 antibody; Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 antibody; ENPP3 antibody ENPP3_HUMAN antibody; gp130RB13 6 antibody; gp130RB136 antibody; M6S1 antibody; NPP1 antibody; NPP3 antibody; NPPase antibody; NPPS antibody; Nucleotide pyrophosphatase antibody; PC 1 antibody; PC-1 antibody; PCA1 antibody; PD Ibeta antibody; PD-Ibeta antibody; PDNP1 antibody; PDNP3 antibody; Phosphodiesterase I beta antibody; Phosphodiesterase I/nucleotide pyrophosphatase 3 antibody; phosphodiesterase i/nucleotide pyrophosphatase beta antibody, B10; NPP3; PDNP3; CD203c; PD-IBETA

Summary

Ecto-nucleotide pyrophosphatase/phosphodiesterase-I enzymes (E-NPP), a group of type II transmembrane proteins, cleave phosphodiester and phosphosulfate bonds in deoxynucleotides, NAD, and nucleotide sugars. There exist three closely related proteins in mammalian species: E-NPP1 (PC-1), E-NPP2 (PDNP2), and E-NPP-3, also known as CD203c, each expressed in different cells or at different locations in the same cells. Basophils, a type of major proinflammatory effector cells involved in diverse pathologic reactions, exhibit CD203c expression. For instance, CD203c expression increases in response to a variety of allergens, including cat dander, latex, and bee and wasp venom. Hovever, these allergies are not the only condition associated with CD203c upregulation; CD203c upregulation may also serve as a tumor marker for colon carcinoma.

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