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CD100 (Semaphorin-4D) Antibody [133-1C6]

In Stock
Catalog Number Formulation Size Price
10507-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
10507-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
10507-MSM1-P1BX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
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Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml

Summary

Recognizes a homodimeric protein comprised of 50kDa subunits, identified as CD100 (Workshop VI; Code N-L026). It is expressed on majority of haemopoietic cells (B, T, NK and myeloid cells) and is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. Its expression is increased after PHA-activation. CD100 was shown to associate with different partner molecules in T cells such as CD45, a key molecule with protein tyrosine phosphatase activity involved in T-cell transduction, and a Serine kinase. It plays a role in homotypic cell adhesion and in T cell activation.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence
Species Reactivity
Baboon, Human
Research Areas
Autophagy, Developmental Biology
Isotype / Light Chain
IgM / Kappa
Cellular Localization
Cell membrane
Gene Name
SEMA4D
Positive Control
Daudi, HUT-78, Kg1a, Raji, U937 and human lymphocytes. Human tonsils and lymph nodes.
Immunogen
PHA stimulated human peripheral blood lymphocytes.
Alternate Names
Semaphorin-4D, A8, BB18, GR3, COLL4; Collapsin4; GR3; M sema G; MSEMA; SEMA4D; Semacl2; SemaH; SEMAJ; Semaphorin C like 2; Semaphorin H; Semaphorin J; Semaphorin-4D; Semcl2

Database Links

Entrez Gene ID
10507
SwissProt
Q92854

Additional Information

Clone
133-1C6
Chromosome Location
9q22.2
Mol. Weight of Antigen
50kDa (Monomer)

Related Antibodies

View All SEMA4D Antibodies

Functions

  • Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling (PubMed:20877282). Regulates GABAergic synapse development (By similarity). Promotes the development of inhibitory synapses in a PLXNB1-dependent manner (By similarity). Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA (PubMed:19788569). Promotes the migration of cerebellar granule cells (PubMed:16055703). Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro) (PubMed:8876214). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway (PubMed:16055703).

Key References

  • Schlossman SF et al. (eds) Leucocyte Typing V Oxford University Press, Oxford. 1995.
  • Kishimoto T et al. (eds) Leukocyte Typing VI, Garland Publishing, New York, 1997.
  • Dorfman DM et al. The leukocyte semaphorin CD100 is expressed in most T-cell, but few B-cell, non-Hodgkin's lymphomas. Am J Pathol 1998, 153(1):255-262.
  • Delaire S et al. CD100 is a leukocyte semaphorin. Cell Mol Life Sci 1998, 54(11):1265-1276.
  • Herold C et al. CD100 is associated with CD45 at the surface of human T lymphocytes. Role in T cell homotypic adhesion. J Immunol 1996, 157(12):5262-5268.
  • Elhabazi A et al. The human semaphorin-like leukocyte cell surface molecule CD100 associates with a serine kinase activity. J Biol Chem 1997, 272(38):23515-23520.
  • Hall KT et al. Human CD100, a novel leukocyte semaphorin that promotes B-cell aggregation and differentiation. Proc Natl Acad Sci USA 1996, 93(21):11780-11785

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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