| Catalog Number: | 567-MSM3 |
|---|---|
| Reactivity: | |
| Gene: | |
| Host: |
Trial Size Available
Carrier-Free Available
Immunofluorescent staining of HeLa cells. Beta-2-Microglobulin Mouse Monoclonal Antibody (BBM.1) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)
Formalin-fixed, paraffin-embedded human kidney stained with Beta-2-microglobulin Mouse Monoclonal Antibody (BBM.1). Inset: PBS instead of primary antibody; secondary only negative control.
Flow Cytometric Analysis of PFA-fixed HeLa cells using Beta-2-Microglobulin Mouse Monoclonal Antibody (BBM.1); followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).
Western Blot Analysis of human THP-1 and Raji cell lysate using Beta-2-Microglobulin Mouse Monoclonal Antibody (BBM.1).
SDS-PAGE Analysis of Purified Beta-2-Microglobulin Mouse Monoclonal Antibody (BBM.1). Confirmation of Integrity and Purity of Antibody.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Flow Cytometry (Flow) | 1-2ug/million cells | Flow Cytometry Protocol | |
| Immunofluorescence (IF) | 1-3ug/ml | ||
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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Recognizes a protein of 12kDa, identified as microglobulin. Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an alpha heavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha3 subdomain of the alpha heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The alpha1 and alpha2 domains of the alpha heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.
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