Flow Cytometric Analysis of PFA-fixed HeLacells. ATF2 Mouse Monoclonal Antibody (PCRP-ATF2-1B4) followed bygoat anti-mouse IgG-CF488 (blue); unstained cells (red).

Immunofluorescence Analysis of HeLacells stained using ATF2 Mouse Monoclonal Antibody (PCRP-ATF2-1B4) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

Flow Cytometric Analysis of PFA-fixed U87cells. ATF2 Mouse Monoclonal Antibody (PCRP-ATF2-1B4) followed bygoat anti-mouse IgG-CF488 (blue); unstained cells (red).

SDS-PAGE Analysis. Purified ATF2 Mouse Monoclonal Antibody (PCRP-ATF2-1B4). Confirmation of Integrity and Purity of Antibody.

Analysis of Protein Array containing more than 19,000 full-length human proteins using ATF2 Mouse Monoclonal Antibody (PCRP-ATF2-1B4). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Printable Datasheet

ATF2 [PCRP-ATF2-1B4]

Name: ATF2
SKU: 1386-MSM2
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies

Summary

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene.

Product Properties & Targets

Host

Mouse

Applications

Flow Cytometry, Immunofluorescence, Western Blot

Species Reactivity

Human

Isotype

IgG2b

Cellular Localization

Cytoplasm, Mitochondrion outer membrane, Nucleus

Gene Name

ATF2

Positive Control

HeLa or U87 cells.

Immunogen

Recombinant full-length human ATF2 protein

Alternate Names

Cyclic AMP-dependent transcription factor ATF-2, Activating transcription factor 2, Cyclic AMP-responsive element-binding protein 2, HB16, cAMP response element-binding protein CRE-BP1, HB16; CREB2; TREB7; CREB-2; CRE-BP1

Database Links

Entrez Gene ID

1386

Unigene

592510

SwissProt

P15336

Additional Information

Clone

PCRP-ATF2-1B4

Human Gene Symbol

ATF2

Chromosome Location

2q31.1

Mol. Weight of Antigen

54.5kDa

Functions

Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro (PubMed:10821277). In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.

Known Applications & Suggested Dilutions

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
,Immunoprecipitation (1-2ug per 100-500ug of total protein)
Western Blot (1-2ug/ml)
,Optimal dilution for a specific application should be determined.

Key References

Venkataraman A, et al. A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors. Nat Methods. 2018;15(5):330-338.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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