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ATF2 Antibody [PCRP-ATF2-1B4]

In Stock
Catalog Number Formulation Size Price
1386-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
1386-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
1386-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
ATF2 Antibody

Flow Cytometric Analysis of PFA-fixed HeLacells. ATF2 Mouse Monoclonal Antibody (PCRP-ATF2-1B4) followed bygoat anti-mouse IgG-CF488 (blue); unstained cells (red).

ATF2 Antibody - Image 2

Immunofluorescence Analysis of HeLacells stained using ATF2 Mouse Monoclonal Antibody (PCRP-ATF2-1B4) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

ATF2 Antibody - Image 3

Immunofluorescence Analysis of HeLacells stained using ATF2 Mouse Monoclonal Antibody (PCRP-ATF2-1B4) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

ATF2 Antibody - Image 4

SDS-PAGE Analysis. Purified ATF2 Mouse Monoclonal Antibody (PCRP-ATF2-1B4). Confirmation of Integrity and Purity of Antibody.

ATF2 Antibody - Image 5

Flow Cytometric Analysis of PFA-fixed U87cells. ATF2 Mouse Monoclonal Antibody (PCRP-ATF2-1B4) followed bygoat anti-mouse IgG-CF488 (blue); unstained cells (red).

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, Cytokine Signaling, MAPK Signaling, Nuclear Marker, Signal Transduction, Stem Cell Differentiation, Transcription Factors
Isotype / Light Chain
IgG2b /
Cellular Localization
Cytoplasm, Mitochondrion outer membrane, Nucleus
Gene Name
ATF2
Positive Control
HeLa or U87 cells.
Immunogen
Recombinant full-length human ATF2 protein
Alternate Names
Cyclic AMP-dependent transcription factor ATF-2, Activating transcription factor 2, Cyclic AMP-responsive element-binding protein 2, HB16, cAMP response element-binding protein CRE-BP1, HB16; CREB2; TREB7; CREB-2; CRE-BP1

Database Links

Entrez Gene ID
1386
SwissProt
P15336

Additional Information

Clone
PCRP-ATF2-1B4
Chromosome Location
2q31.1
Mol. Weight of Antigen
54.5kDa

Related Antibodies

View All ATF2 Antibodies

Functions

  • Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro (PubMed:10821277). In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.

Key References

  • Venkataraman A, et al. A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors. Nat Methods. 2018;15(5):330-338.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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