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Home >> Antibodies >> Vimentin (Mesenchymal Cell Marker)

Vimentin (Mesenchymal Cell Marker)

Mouse Monoclonal Antibody [Clone VM452]

Catalog #

Size

0.5 ml at 100ug/ml

Price (USD)

459.00

Formulation

Purified Ab conjugated to CF488

Catalog No

Size

0.5 ml at 100ug/ml

Formulation

Purified Ab conjugated to CF488

Price (USD)

459.00
img

Flow Cytometry of human Vimentin on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: CF488-labeled Vimentin Monoclonal Antibody (VM452).

Product Details

Synonyms

VIM

Positive Control

Jurkat cells. Sarcomas or melanomas.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood)
Immunofluorescence (1:50-1:100)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human vimentin protein

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Cow. Dog. Cat. Pig. Goat. Chicken. Does not react with Mouse or Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

57-60kDa

Specificity & Comments

This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

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Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.

Key References

  1. Osborn M et. al. European Journal of Cell Biology. 1984; 34:137-143.

Bioinformatics

  •  Entrez Gene ID: 7431
  •  Gene Symbol: VIM
  •  SwissProt: P08670
  •  Chromosome Location: 10p12.33
  •  Unigene: 455493
NBT00452