Formalin-fixed, paraffin-embedded human tonsil stained with Vimentin Recombinant Rabbit Monoclonal Antibody (VIM/6430R).

SDS-PAGE Analysis Purified Vimentin Recombinant Rabbit Monoclonal Antibody (VIM/6430R). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of U87 cell lysate using Vimentin Recombinant Rabbit Monoclonal Antibody (VIM/6430R).

Formalin-fixed, paraffin-embedded feline (cat) kidney stained with Vimentin Recombinant Rabbit Monoclonal Antibody (VIM/6430R).

Formalin-fixed, paraffin-embedded canine (dog) kidney stained with Vimentin Recombinant Rabbit Monoclonal Antibody (VIM/6430R).

Formalin-fixed, paraffin-embedded bovine (cow) brain stained with Vimentin Recombinant Rabbit Monoclonal Antibody (VIM/6430R).

Formalin-fixed, paraffin-embedded equine (horse) small intestine stained with Vimentin Recombinant Rabbit Monoclonal Antibody (VIM/6430R).

Formalin-fixed, paraffin-embedded equine (horse) lung stained with Vimentin Recombinant Rabbit Monoclonal Antibody (VIM/6430R).

Vimentin (Mesenchymal Cell Marker) [VIM/6430R]

Name: Vimentin (Mesenchymal Cell Marker)
SKU: 7431-RBM10
Availability: InStock

Monoclonals Primary Antibodies Rabbit Monoclonals Recombinant Rabbit Monoclonals Recombinants

Summary

This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

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Product Properties & Targets

Host

Rabbit

Species Reactivity

Cat, Chicken, Cow, Dog, Horse, Human, Pig, Rat

Isotype

IgG

Cellular Localization

Cell membrane, Cytoplasm, Cytoskeleton, Nucleus matrix

Gene Name

VIM

Mol. Weight of Antigen

57-60kDa

Immunogen

Recombinant fragment (around aa 2-466) of human Vimentin protein (exact sequence is proprietary)

Database Links

Entrez Gene ID

7431

Unigene

455493

SwissProt

P08670

Additional Information

Clone

VIM/6430R

Human Gene Symbol

VIM

Chromosome Location

10p12.33

Known Applications & Suggested Dilutions

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

Osborn M, et. al. European Journal of Cell Biology. 1984; 34:137-143.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.