Vimentin (Mesenchymal Cell Marker) [VM452]

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Summary

This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Product Properties & Targets

Host
Research Areas
Isotype
Cellular Localization
cytoplasmic
Gene Name
VIM
Positive Control
Jurkat cells. Sarcomas or melanomas.
Immunogen
Recombinant human vimentin protein
Alternate Names
Vimentin, VIM

Database Links

Entrez Gene ID
Unigene
SwissProt

Additional Information

Clone
VM452
Human Gene Symbol
VIM
Chromosome Location
10p12.33
Mol. Weight of Antigen
57-60kDa

Functions

  • Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
  • Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Known Applications & Suggested Dilutions

  • Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood);Immunofluorescence (1:50-1:100);Optimal dilution for a specific application should be determined.

Key References

  • Osborn M et. al. European Journal of Cell Biology. 1984; 34:137-143.

PubMed Links

Storage & Stability

Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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