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Home >> Antibodies >> Thymidylate Synthase (5-FU Resistance Marker)

Thymidylate Synthase (5-FU Resistance Marker)

Mouse Monoclonal Antibody [Clone TYMS/1884]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Tonsil stained with Thymidylate Synthase Mouse Monoclonal Antibody (TYMS/1884).

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SDS-PAGE Analysis Purified Thymidylate Synthase Mouse Monoclonal Antibody (TYMS/1884). Confirmation of Purity and Integrity of Antibody.

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Western Blot Analysis of human HeLa cell lysate using Thymidylate Synthase Mouse Monoclonal Antibody (TYMS/1884).

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Immunofluorescence Analysis of PFA-fixed Ramos cells labeling PU.1 with Thymidylate Synthase Mouse Monoclonal Antibody (TYMS/1884) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is labeled with Phalloidin (Red).

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Flow Cytometric Analysis of PFA-fixed MOLT4 cells. Thymidylate Synthase Monospecific Mouse Monoclonal Antibody followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

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Flow Cytometric Analysis of PFA-fixed Ramos cells. Thymidylate Synthase Monospecific Mouse Monoclonal Antibody followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Thymidylate Synthase Monospecific Mouse Monoclonal Antibody (TYMS/1884)

Product Details

Synonyms

dTMP synthase, TMS, TS, TSase, TYMS protein, Tyms thymidylate synthetase

Positive Control

MOLT4; Ramos, 5-FU-resistant colon carcinoma cell lines (NCI H630R10, NCI H630R1); 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10. Testicular carcinomas.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human thymidylate synthase protein fragment (around aa 60-174) (exact sequence is proprietary)

Cellular Localization

Nuclear & Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2c, kappa

Mol. Weight of Antigen

36kDa

Specificity & Comments

It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Key References

  1. Pestalozzi BC, et. al. Journal of Clinical Oncology, 1997, 15(5):1923-31
  2. Johnston PG, et. al. Journal of the National Cancer Institute, 1997, 89(4):308-13

Bioinformatics

  •  Entrez Gene ID: 7298
  •  Gene Symbol: TYMS
  •  SwissProt: P04818
  •  Chromosome Location: 18p11.32
  •  Unigene: 369762
NBT01884