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Formalin-fixed, paraffin-embedded human Tonsil stained with Thymidylate Synthase Mouse Monoclonal Antibody (TYMS/1884).
SDS-PAGE Analysis Purified Thymidylate Synthase Mouse Monoclonal Antibody (TYMS/1884). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of human HeLa cell lysate using Thymidylate Synthase Mouse Monoclonal Antibody (TYMS/1884).
Immunofluorescence Analysis of PFA-fixed Ramos cells labeling PU.1 with Thymidylate Synthase Mouse Monoclonal Antibody (TYMS/1884) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is labeled with Phalloidin (Red).
Flow Cytometric Analysis of PFA-fixed MOLT4 cells. Thymidylate Synthase Monospecific Mouse Monoclonal Antibody followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Flow Cytometric Analysis of PFA-fixed Ramos cells. Thymidylate Synthase Monospecific Mouse Monoclonal Antibody followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Analysis of Protein Array containing more than 19,000 full-length human proteins using Thymidylate Synthase Monospecific Mouse Monoclonal Antibody (TYMS/1884) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDÂ’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDÂ’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Flow Cytometry (Flow) | 1-2ug/million cells | Flow Cytometry Protocol | |
| Immunofluorescence (IF) | 1-3ug/ml | ||
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
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