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Thymidylate Synthase (5-FU Resistance Marker) Antibody [SPM453]

In Stock
Catalog Number Formulation Size Price
7298-MSM1X-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
7298-MSM1X-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
7298-MSM1X-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Research Areas
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm, Mitochondrion, Mitochondrion inner membrane, Mitochondrion matrix, Nucleus
Gene Name
Positive Control
5-FU-resistant colon carcinoma cell lines (NCI H630R10, and breast carcinomas., gastric, head & neck, MCF-Ad5 and MCF-Ad10. Colorectal, NCI H630R1); 5-FU-resistant breast cancer cell lines
Immunogen
Recombinant full-length human TYMS protein.
Alternate Names
Thymidylate synthase, dTMP synthase, TMS, TS, TSase, TYMS protein, Tyms thymidylate synthetase

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
SPM453
Chromosome Location
18p11.32
Mol. Weight of Antigen
36kDa

Functions

  • Catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1-carbon donor and reductant and contributes to the de novo mitochondrial thymidylate biosynthesis pathway.

Key References

  • Johnston PG, et. al. Cancer Research, 1991, 51(24):6668-76.
  • Johnston PG, et. al. Cancer Research, 1992, 52(16):4306-12.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. ,Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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