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Recombinant Thymidylate Synthase (5-FU Resistance Marker) Antibody [rTYMS/1884]

In Stock
Catalog Number Formulation Size Price
7298-MSM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
7298-MSM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
7298-MSM6-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml

Summary

It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

Product Properties & Targets

Host
Mouse
Species Reactivity
Research Areas
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm, Mitochondrion, Mitochondrion inner membrane, Mitochondrion matrix, Nucleus
Gene Name
Positive Control
MOLT-4 or Ramos cells. Testicular carcinomas.
Immunogen
Recombinant human thymidylate synthase protein fragment (around aa 60-174) (exact sequence is proprietary)
Alternate Names
Thymidylate synthase, dTMP synthase, TMS, TS, TSase, TYMS protein, Tyms thymidylate synthetase

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
rTYMS/1884
Chromosome Location
18p11.32
Mol. Weight of Antigen
36kDa

Functions

  • Catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1-carbon donor and reductant and contributes to the de novo mitochondrial thymidylate biosynthesis pathway.

Key References

  • Pestalozzi BC, et. al. Journal of Clinical Oncology, 1997, 15(5):1923-31
  • Johnston PG, et. al. Journal of the National Cancer Institute, 1997, 89(4):308-13

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. ,Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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