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Home >> Antibodies >> Thymidylate Synthase (5-FU Resistance Marker)

Thymidylate Synthase (5-FU Resistance Marker)

Mouse Monoclonal Antibody [Clone SPM453]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

Formalin-fixed, paraffin-embedded human testicular carcinoma stained with Thymidylate Synthase Mouse Monoclonal Antibody (SPM453).

Product Details

Synonyms

dTMP synthase, TMS, TS, TSase, TYMS protein, Tyms thymidylate synthetase

Positive Control

5-FU-resistant colon carcinoma cell lines (NCI H630R10, NCI H630R1); 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10. Colorectal, gastric, head & neck, and breast carcinomas.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human TYMS protein.

Cellular Localization

Cytoplasm. Nucleus. Mitochondria.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

36kDa

Specificity & Comments

It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Key References

  1. Johnston PG, et. al. Cancer Research, 1991, 51(24):6668-76.
  2. Johnston PG, et. al. Cancer Research, 1992, 52(16):4306-12.

Bioinformatics

  •  Entrez Gene ID: 7298
  •  Gene Symbol: TYMS
  •  SwissProt: P04818
  •  Chromosome Location: 18p11.32
  •  Unigene: 369762