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Home >> Antibodies >> Thomsen-Friedenreich Antigen / CD176 (Pan Carcinoma Marker)

Thomsen-Friedenreich Antigen / CD176 (Pan Carcinoma Marker)

Mouse Monoclonal Antibody [Clone A63-C/A9]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with T-F Antigen Mouse Monoclonal Antibody (A63-C/A9).

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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with T-F Antigen Mouse Monoclonal Antibody (A63-C/A9).

Product Details

Synonyms

T-F Antigen; TF Antigen; Asialoglycophorin; pan-carcinoma marker; CD176

Positive Control

KG1 cells. Human colorectal carcinoma tissues.

Known Applications & Suggested Dilutions

IF, IHC-Fr, IHC-P         More Details

Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Frozen) (1-2ug/ml for 30 minutes at RT)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at 37'C)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Neuraminidase-treated human red blood cells

Cellular Localization

Cell Surface

Species Reactivity

Human. Mouse. Rat.

Host / Ig Isotype

Mouse / IgM, kappa

Mol. Weight of Antigen

Multiple

Specificity & Comments

Recognizes a disaccharide epitope, Gal 1-3GalNAc, of Thomsen-Friedenreich (TF) antigen. It is specific for both anomeric forms of the disaccharide (TF and TF , including related structures on the glycolipid) and shows no cross-reactivity with sialylated glycophorin. The Thomsen-Friedenreich antigen acts as an oncofetal antigen, with low expression in normal adult tissues but increasing to fetal levels of expression in hyperplasia or malignancy. It is considered as a pan-carcinoma marker. This MAb is capable to agglutinate desialylated red blood cells. During metastasis, the ability of malignant cells to form multicellular aggregates via homotypic or heterotypic aggregation and their adhesion to the endothelium are critical. The tumor-associated carbohydrate Thomsen-Friedenreich antigen (Gal-GalNAc) is involved in tumor cell adhesion and tissue invasion. It also causes an immune response, and overexpression of the antigen causes cancer cells to be more sensitive to natural killer cell lysis. The Thomsen-Friedenreich antigen is suppressed in normal healthy cells and represents one of the few chemically well-defined antigens associated with tumor malignancy. The presence of the Thomsen-Friedenreich antigen on the surface of cancer cells may result from a divergence from the normal pathway for O-linked glycosylation in these cells, most likely caused by inappropriate localization of the enzymes involved in synthesis of the disaccharide.

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200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Baldus SE et al. Cancer 1998, 82(6):1019-27
  2. Karsten U et al. Hybridoma 1995, 14(1):37-44
  3. Ryder SD et al. Gastroenterology 1998, 114(1):44-9

Bioinformatics

NBT00938