Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with RNA Polymerase II / Poll II Mouse Monoclonal Antibody (CTD4H8).
SDS-PAGE Analysis Purified RNA Poll II Mouse Monoclonal Antibody (CTD4H8). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of Human HAP1, K562, PC3, HePG2 & NIH3T3 cell lysates using RNA Poll II Mouse Monoclonal Antibody (CTD4H8).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.