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RNA Polymerase II

Mouse Monoclonal Antibody [Clone CTD4H8]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with RNA Polymerase II / Poll II Mouse Monoclonal Antibody (CTD4H8).

SDS-PAGE Analysis Purified RNA Poll II Mouse Monoclonal Antibody (CTD4H8). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of Human HAP1, K562, PC3, HePG2 & NIH3T3 cell lysates using RNA Poll II Mouse Monoclonal Antibody (CTD4H8).

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with RNA Polymerase II / Poll II Mouse Monoclonal Antibody (CTD4H8).

SDS-PAGE Analysis Purified RNA Poll II Mouse Monoclonal Antibody (CTD4H8). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of Human HAP1, K562, PC3, HePG2 & NIH3T3 cell lysates using RNA Poll II Mouse Monoclonal Antibody (CTD4H8).

Product Details

Synonyms

DNA-directed RNA polymerase II largest subunit RNA polymerase II 220kd subunit; DNA-directed RNA polymerase II subunit RPB1; hRPB220; hsRPB1; POLR2; Polr2a; POLRA; Polymerase (RNA) II (DNA directed) polypeptide A 220kDa; RNA polymerase II subunit B1; RPBh1; RpIILS; RPO2; RPOL2

Positive Control

HAP1, K562, PC3, HePG2, NIH3T3 cells. Human testis.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Ten repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5

Cellular Localization

Nuclear

Species Reactivity

Human. Mouse. Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

192-253kDa

Specificity & Comments

RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.